MOLECULAR DIVERSITY OF RUMEN METHANOGENS IN CATTLE IN RESPONSE TO DIETARY TANNIN

Gondelina A. Radovan, Cesar C. Sevilla, Severino S. Capitan, Renato S. A. Vega, Antonio J. Alcantara, Medino Gedeun N. Yebron

Abstract


Philipp J Vet Anim Sci 2013, 39 (1): 21-30


The molecular diversity of rumen methanogens in cattle fed tanninrich banana leaves or supplemented with commercial tannin extract was assessed using PCR-DGGE. Primer set 0357 F-GC and 0691 R was used to amplify the methanogenic archaeal community of the rumen. A total of 17 DNA fragments were excised from DGGE gels and their nucleotide sequences were determined. PCR-DGGE band profile and nucleotide sequence analysis revealed that methanogen resembling Methanobrevibacter thaueri strain CW and Methanobrevibacter millerae strain ZA-10 are the predominant methanogens in cattle. Furthermore, feeding of tannin-rich banana leaves to cattle significantly increased blood urea nitrogen but decreased rumen fluid ammonia nitrogen (RF NH3-N) level suggesting a protective effect of tannin on
feed protein and possible inhibitory effect on methanogens by limiting the supply of available hydrogen from ammonia.


Keywords


banana leaves; cattle; methanogens; rumen; tannin

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